Vimentin Antibody (Center)

• Catalog Number: orb1931501
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-181 amino acids from the Central region of human Vimentin.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Hamster, Mouse, Porcine, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P08670
• MW: 53652 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB15676
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Vimentin, VIM
• Research Area: Cancer Biology, Infectious Disease & Virology, Neu
• Note: For research use only
• NCBI: NP_003371.2
• Expiration Date: 12 months from date of receipt.

Vimentin Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Vimentin Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of Vimentin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Western blot analysis of Vimentin Antibody (Center) in A2058, A375 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified Pab.

Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2).