TIGIT Single Domain Antibody [2C7]

• Catalog Number: orb1274206
• Category: Antibodies
• Description: TIGIT Single Domain Antibody [2C7]
• Target: TIGIT
• Clonality: Recombinant
• Isotype: sdAb
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: TIGIT Antibody is supplied in PBS.
• Purification: TIGIT Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
• Immunogen: TIGIT antibody was raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.
• UniProt ID: Q495A1
• Tested applications: ELISA, FC, ICC, IF, IHC-P
• Application notes: TIGIT antibody can be used for ELISA starting at 0.1 μg/mLimmunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. For flow cytometry at 1 μg/ml. For immunocytochemistry at 10μg/mL.
• Antibody Type: Primary Antibody
• Clone Number: 2C7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Alternative names: TIGIT Antibody: T-cell immunoreceptor with Ig and
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: NP_776160
• Expiration Date: 12 months from date of receipt.

Titration ELISA analysis of TIGIT sdAbs to detect recombinant TIGIT (extracellular domain) coated at 1 ug/mL. sdAbs are detected with a mouse mAb against a C-terminal myc-tag followed by a goat anti-mouse IgG-HRP conjugate.

Immunohistochemistry of TIGIT in human spleen tissue with TIGIT single domain antibody at 1 μg/mL.

Flow cytometry analysis of TIGIT overexpressing HEK293 cells using TIGIT single domain antibody and control 1H4 single domain antibody at 0.05 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIGIT overexpressing HEK293 cells.

Immunofluorescence of TIGIT in human spleen tissue with TIGIT single domain antibody at 20 μg/mL.

Immunocytochemistry of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 10 μg/mL.

Immunofluorescence of TIGIT in transfected HEK293 cells with TIGIT single domain antibody at 20 μg/mL.