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| Catalog Number | orb770253 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to TGF beta RI (phospho-Ser165) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I around the phosphorylation site of Ser165. AA range:131-180 |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-2000, IF 1:50-300, IHC 1:50-300 |
| Conjugation | Unconjugated |
| MW | 56kD |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-TGFBR1 antibody, anti-ALK5 antibody, anti-SKR Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, TGFβ RI (phospho Ser165) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western Blot analysis of MOUSE-LIVER cells using Phospho-TGFβ RI (S165) Polyclonal Antibody diluted at 1:1000.
