TAG-72 Antibody [B72.3]

SKU: orb1252648

Description

TAG-72 Antibody [B72.3]

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC
• Reactivity: Bovine, Canine, Hamster, Human, Rat
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: B72.3
• Immunogen: The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of human ovarian carcinoma with TAG-72 antibody.

IHC staining of human prostate carcinoma with TAG-72 antibody.