STAT3 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559148
• Category: Antibodies
• Description: STAT3 Recombinant Rabbit Monoclonal Antibody
• Target: STAT3
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Zebrafish
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: Recombinant human Stat3 protein (1-100aa)
• UniProt ID: P40763
• MW: 88 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:20-100
• Antibody Type: Recombinant Antibody
• Clone Number: 6G5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 1110034C02Rik; Acute Phase Response Factor; APRF;
• Note: For research use only

25 ug total protein per lane of various lysates probed with STAT3 monoclonal antibody, unconjugated (orb559148) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

25 ug total protein per lane of various lysates probed with STAT3 monoclonal antibody, unconjugated (orb559148) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-Stat3 antibody (orb559148), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.