SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse Monoclonal Antibody

• Catalog Number: orb638870
• Category: Antibodies
• Description: SARS-CoV-2 (2019-nCoV) Nucleocapsid Mouse Monoclonal Antibody
• Target: S
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Virus
• Predicted Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: ≥1 mg/ml
• Buffer/Preservatives: 0.01M PBS (pH7.4) with 0.02% Proclin300.
• Purification: Affinity purified by Protein G
• Immunogen: Recombinant SARS-CoV-2 Nucleocapsid Protein (1-419/419aa)
• MW: 46 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Clone Number: 1C7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nu
• Research Area: SARS /COVID-19
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Christy J Sadler 1 2, Jan P Sandler 1, André Shamsabadi 1 2, Leah C Frenette 1, Adam Creamer 1 2, Molly M Stevens Signal Enhancement in Immunoassays via Coupling to Catalytic Nanoparticles ACS Sens ., (2025)

PubMed: 40390533

Sample: Lane 1: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 100 ng, Lane 2: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 50 ng, Lane 3: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 25 ng, Lane 4: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 10 ng, Lane 5: Recombinant SARS-CoV-2 N Protein (Omicron (B.1.1.529) (P13L, E31del, R32del, S33del, R203K, G204R) at 5 ng, Lane 6: Recombinant SARS-CoV-2 N Protein (WT) at 10 ng, Primary: Mouse Anti-SARS-CoV-2 N protein (orb638870) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 45.3 kD, Observed band size: 50 kD.

Sample: Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag), Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag), Lane 3: Recombinant SARS-CoV-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F) (His Tag), Lane 4: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag), Lane 5: Recombinant SARS-Cov-2 N protein (R203M, D377Y) (His Tag), Lane 6: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag), Primary: Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid (orb638870) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 48 kDa.

Sample: Lane 1: SARS-CoV-2 N protein (orb1516719) at 50 ng, Lane 2: SARS-CoV-2 N protein (orb1516719) at 25 ng, Lane 3: SARS-CoV-2 N protein (orb1516719) at 5 ng, Lane 4: SARS-CoV-2 N protein (orb1516719) at 2 ng, Lane 5: SARS-CoV-2 N protein (orb1516719) at 1 ng, Lane 6: SARS-CoV-2 N protein (orb1516719) at 0.5 ng, Primary: Anti-SARS-CoV-2 N protein at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46 kD, Observed band size: 46 kD.