Rhodamine-12-dCTP

SKU: orb533143

Description

Rhodamine-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Rhodamine-12-dCTP/dCTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dCTP/70-50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 505 nm, λem 530 nm, ε 85.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 989.71 g/mol (free acid); Detected MW: 989.18 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₉H₄₂N₇O₁₈P₃
• SMILES: N(CC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)C(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2ccc(cc2oc2-c1ccc(=[NH2+])c2)N)C(=O)[O-]

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only