TP53 Antibody

SKU: orb1274258

Description

TP53 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Reactivity: Human
• Application Notes:
  Western blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.25-0.5 µg/mL for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG, kappa
• Clone No.: TP53/1799R
• Immunogen: Recombinant human p53 protein was used as the immunogen for this recombinant p53 antibody.
• Target: TP53
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Analysis of HuProt(TM) microarray containing more than 19000 full-length human proteins using recombinant p53 antibody (clone TP53/1799R). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC testing of FFPE human colon carcinoma with recombinant p53 antibody (clone TP53/1799R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human HeLa cell lysate with recombinant p53 antibody (clone TP53/1799R).

Quick Database Links

Gene Symbol

TP53

UniProt

P04637