Recombinant p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Antibody

• Catalog Number: orb750918
• Category: Antibodies
• Description: Mouse monoclonal antibody against p63 conjugated to Biotin
• Target: TP63
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Immunogen: A synthetic peptide from the N-terminal of human p40 protein (Exact sequence is proprietary)
• UniProt ID: Q9H3D4
• MW: 40kDa
• Tested applications: FC, IF, IHC
• Dilution range: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HeLa cells. Human prostate or lung tissue (IHC)
• Antibody Type: Recombinant Antibody
• Clone Number: rTP40/3690
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-deltaNp63 antibody, anti-TP63 antibody
• Research Area: Cell Biology, Epigenetics & Chromatin, Stem Cell &
• Note: For research use only
• Entrez: 8626
• Expiration Date: 12 months from date of receipt.

Flow Cytometric Analysis of PFA-fixed HeLa cells using p40 Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human prostate stained with Biotinylated p40 Recombinant Mouse Monoclonal Antibody (rTP40/3690).