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Catalog Number | orb2658865 |
---|---|
Category | Proteins |
Description | Recombinant Macaca fascicularis G protein-coupled receptor 20 (GPR20)-VLPs (Active) |
Tag | C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions) |
Form/Appearance | Lyophilized powder |
Purity | The purity information is not available for VLPs proteins. |
MW | 40.2 kDa |
UniProt ID | XP_015310747.1 |
Protein Sequence | MPSVSPVGPSAGAVPNATAVTTVWTNASGLEVPLFHLFARLDEELHGTFPGLWLALMAVHGAIFLVGLVLNGLALYVFCCRTQAKTPSVIYTINLVVTDLLVGLSLPTRFAVYYGARGCLHCAFPHVLGYFLNMHCSILFLTCICVDRYLAIVRPEGSRRCRQPACARAVCAFVWLAAGAVTLSVLGMTGGRPCCRVFALTVLEFLLPLLVISVFTGRIMCALSRPGLLRQGRQRRVRAMQLLLTVLIIFLVCFTPFHARQVAVALWPDMPHHASLVVYHVAVTLSSLNSCMDPIVYCFVTSGFQATVRGLFGQHRGEREPSSGDVVSMHRSSKGSGRHHILSAGPHALTQALANGPEA |
Protein Length | Full Length |
Source | Mammalian cell |
Biological Origin | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPR20 at 10 μg/mL can bind Anti-GPR20 recombinant antibody(CSB-RA860774MA1HU). The EC50 is 3.549 - 6.542 ng/mL.The VLPs (CSB-MP3838) is negative control. |
Expression Region | 1-359aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing. |
Expiration Date | 6 months from date of receipt. |
Detected by Mouse anti-6*His monoclonal antibody. (This tag can be tested only under denaturing conditions.)
Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis GPR20 at 10 μg/ml can bind Anti-GPR20 recombinant antibody. The EC50 is 3.549 - 6.542 ng/mL.The VLPs is negative control.
The purity of VLPs was greater than 95% as determined by SEC-HPLC