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| Catalog Number | orb2657853 |
|---|---|
| Category | Antibodies |
| Description | Recombinant human IFN gamma Antibody |
| Clonality | Recombinant |
| Conjugation | Unconjugated |
| Purification | Purified by affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0). |
| Tested applications | ELISA, Functional Studies |
| Application notes | Recombinant protein IFN gamma is expressed in S2 cell (Drosophila). DNA sequence encoding 143 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18 kDa.ELISA: Recombinant protein used as a standard in ELISA may not fully reflect the properties of the native protein, which can lead to inaccurate quantification of its concentration in samples. |
| Antibody Type | Recombinant Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
| Entrez | 35 |

Recombinant Human IFN gamma induces cytotoxicity in HT-29 human colon adenocarcinoma cell line. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 71.5 ng/ml.

Recombinant Human IFN gamma induces cytotoxicity in HT-29 human colon adenocarcinoma cell line. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 71.5 ng/ml.
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