RBP1/CRBP Antibody

• Catalog Number: orb1538100
• Category: Antibodies
• Description: RBP1/CRBP Antibody
• Target: RBP1 / CRBP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Monkey
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence VEGVVCKQVFKKVQ, from the C-Terminus of protein sequence according to NP_002890.2.
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Dilution range: IF (10 µg/ml), IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:4000), WB (0.5 - 3 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B3600 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3600 was determined to be 5 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RBP1, CRABP-I, CRBP, CRBP-I, RBPC, Retinol-binding
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RBP1 / CRBP antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

RBP1 / CRBP antibody (2 µg/ml) staining of NIH3T3 (A) and (0.5 ug/ml) of U251 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

RBP1 / CRBP antibody flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.