Rat IgG2a isotype control Fluorescein Antibody

• Catalog Number: orb343817
• Category: Proteins
• Description: Rat IgG2a isotype control Fluorescein Antibody
• Isotype: IgG2a
• Conjugation: FITC
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG1, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Rat IgG2a confirmed by Dot Blot.
• Tested applications: DOT, ELISA, FC
• Dilution range: ELISA: 1:2000 - 1:20,000, FC: 1:1000-1:5000
• Application notes: RAT IgG2a isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.
• Source: Rat
• Biological Origin: Rat
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
• Alternative names: Rat Isotype Control, Rat IgG2a FITC isotype, RAT I
• Research Area: Protein Biochemistry
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rat IgG2a Isotype Control Fluorescein Conjugated. Antigen: Rat IgG2a Isotype Control FITC Conjugated. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT.