Rat Endothelin 2 (EDN2) ELISA Kit

• Catalog Number: orb780117
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Endothelin 2(EDN2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endothelin 2(EDN2).
• Reactivity: Rat
• Concentration: 500 pg/mL
• UniProt ID: P23943
• Tested applications: ELISA
• Application notes: standard: 500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat EDN2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat EDN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat EDN2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.66 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Alternative names: ET2 ELISA Kit, PPET2 ELISA Kit, Preproendothelin-2
• Research Area: Cardiovascular Research
• Note: For research use only
• Expiration Date: Please enquire.