Rat Anti-Thyroglobulin Ab (TG-ab) ELISA Kit

• Catalog Number: orb782152
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroglobulin Ab(TG-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroglobulin Ab(TG-Ab).
• Reactivity: Rat
• Concentration: 100 ng/mL
• Tested applications: ELISA
• Application notes: standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TG-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TG-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TG-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TG-Ab in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 2.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.62 ng/mL
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Alternative names: TG AB ELISA Kit, TG-AB ELISA Kit
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only
• Expiration Date: Please enquire.