Rabbit PTEN Recombinant Monoclonal Antibody

SKU: orb1784572

Description

Rabbit PTEN Recombinant Monoclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: Application : WB , ApplicationDilutionRange : 1:1000
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR270L
• Immunogen: Between 353 and C-terminus
• Target: PTEN
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

10q23del; BZS; CWS1; DEC; GLM2; MHAM; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; protein tyrosine phosphatase; PTEN1; PTENbeta; TEP1

Detection of human PTEN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, OVCAR-3, SR, MCF-7, and NCI-H460 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784572) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse and rat PTEN by western blot. Samples: Whole cell lysate (50 µg) from 70Z/3, NIH 3T3, Ba/F3, EL4, and C6 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PTEN recombinant monoclonal antibody (orb1784572) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Quick Database Links

Gene Symbol

PTEN

UniProt

P60484

RefSeq (Protein):NP_000305.3