Rabbit Phospho RNA Polymerase II (S2) Antibody

SKU: orb1529845

Description

RNA Polymerase II, Phospho (S2) Antibody

Research Area

Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Drosophila
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Surrounding serine 2
• Target: RNA Polymerase II Phospho (S2)
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; hRPB220; hsRPB1; POLR2; POLRA; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Detection of human Phospho RNA Polymerase II (S2) by western blot.Samples: HeLa prepared using NETN buffer. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1529845 was at 0.04 µg/ml (upper panel).

Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2µg/ml).

Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:200 (1µg/ml).

Detection of Phospho RNA Polymerase II (S2) by WB of immunoprecipitates.Samples: HeLa cells.Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody for IP and Affinity purified rabbit anti-RNA Polymerase II antibody for IP.

Quick Database Links

Gene Symbol

RNA Polymerase II Phospho (S2)

UniProt

P24928

RefSeq (Protein):NP_000928.1