Rabbit anti-PD-L1 Recombinant Monoclonal Antibody

• Catalog Number: orb1519952
• Category: Antibodies
• Description: PD-L1 Antibody
• Target: PD-L1
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 50 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Purity: Recombinant antibody was purified from cell culture supernatant
• Immunogen: Between 240 and C-terminus
• UniProt ID: Q9NZQ7
• Tested applications: FC, ICC, IF, IHC, WB
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells. ICC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
• Antibody Type: Primary Antibody
• Clone Number: BLR020E
• Storage: 2 - 8°C
• Alternative names: PDCD1L1; Programmed death ligand 1; programmed cel
• Background: PD-L1 is an immune inhibitory receptor ligand that
• Note: For research use only
• NCBI: NP_054862.1
• Expiration Date: 12 months from date of receipt.

Detection of human PD-L1 by western blot. Samples: Whole cell lysate (50 µg) from RKO, Jurkat, SUP-M2, HEK293T, and HDLM-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human PD-L1 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human PD-L1 (red) and Lamin-A/C (green) in FFPE lung carcinoma by IHC-IF. Antibody: Rabbit anti-PD-L1 recombinant monoclonal and rabbit anti-Lamin-A/C. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG and DyLight® 488-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

Detection of human PD-L1 (green) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Detection of human PD-L1 by immunocytochemistry. Sample: FFPE section of human SUP-M2 cells. Antibody: Rabbit anti-PD-L1 recombinant monoclonal antibody Secondary: HRP-conjugated goat anti-rabbit IgG