Rabbit PARN Antibody

SKU: orb1524595

Description

PARN Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 589 and C-terminus
• Target: PARN
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DAN; deadenylating nuclease; deadenylation nuclease; DKCB6; PFBMFT4; poly(A)-specific ribonuclease PARN; polyadenylate-specific ribonuclease

Detection of mouse PARN by western blot. Samples: Whole cell lysate (10 µg) from CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (orb1524595) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PARN by western blot. Samples: Whole cell lysate (10 µg) from LNCaP, MCF-7, Hep-G2, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PARN antibody (orb1524595) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PARN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PARN antibody (orb1524595) used for IP at 6 µg per reaction. PARN was also immunoprecipitated by a previous lot of this antibody (orb1524595) and a second antibody against a different epitope of PARN.

Detection of human PARN by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PARN (orb1524595) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

PARN

UniProt

O95453

RefSeq (Protein):NP_002573.1