Rabbit anti-p16INK4a Recombinant Monoclonal Antibody

• Catalog Number: orb2281287
• Category: Antibodies
• Description: Rabbit anti-p16INK4a Recombinant Monoclonal Antibody
• Target: p16INK4a
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 500 µg/ml
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Between 106 and C-terminus
• UniProt ID: P42771
• Tested applications: FC, ICC, IHC, IP, WB
• Dilution range: WB: 1:1,000, IP: 12 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR318M
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: P16-INK4A; multiple tumor suppressor 1; P14ARF; P1
• Note: For research use only

Detection of human p16INK4a by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, OVCAR-8, SK-MEL-28, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds

Detection of human p16INK4a by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human p16INK4a by immunocytochemistry. Sample: FFPE section of HEK293T cells. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human p16INK4a (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-p16INK4a recombinant monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG