Rabbit GITR/TNFRSF18 Recombinant Monoclonal Antibody

SKU: orb1519833

Description

GITR/TNFRSF18 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: WB - 1:1000; IP - 6 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR068G
• Immunogen: Between 191 and C-terminus
• Target: GITR/TNFRSF18
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

activation-inducible TNFR family receptor; AITR; CD antigen CD357; CD357; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; tumor necrosis factor receptor superfamily member 18

Detection of human GITR/TNFRSF18 by western blot. Samples: Whole cell lysate (15 or 50 µg) from MJ, Jurkat, RPMI-8226, HeLa, Raji, MOLT-4, Daudi, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-ß-Actin recombinant monoclonal antibody.

Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human GITR/TNFRSF18 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-GITR/TNFRSF18 recombinant monoclonal antibody (orb1519833) used at 1:200. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

GITR/TNFRSF18

UniProt

Q9Y5U5

RefSeq (Protein):NP_004186.1