Rabbit anti-GFP Recombinant Monoclonal Antibody

• Catalog Number: orb1784590
• Category: Antibodies
• Description: Rabbit anti-GFP Recombinant Monoclonal Antibody
• Target: GFP
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Form/Appearance: Whole IgG
• Concentration: 50 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Green Fluorescent Protein
• Tested applications: ICC, IP, WB
• Dilution range: WB: 1:1000, IP: 20 µl/mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLRE04L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of GFP by western blot.Samples: Whole cell lysate from HEK293T cells transfected with EGFP-KAP1, KAP1-EGFP, or untransfected. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.

Detection of GFP by western blot.Samples: Whole cell lysate from 293T cells transfected with EGFP, YP, CFP, AcGFP, or BFP. Antibody: Rabbit anti-GFP recombinant monoclonal antibody used at 1:1000.

Detection of GFP by western blot of immunoprecipitates.Samples: Whole cell lysate from 293-F cells tranfected with EGFP-KAP1. Antibodies: Rabbit anti-GFP recombinant monoclonal antibody.

Detection of GFP by ICC. Sample: FFPE section of HEK293 cells expressing a GFP nuclear protein (left) and untransfected HEK293 cells (right). Antibody: Rabbit anti-GFP recombinant monoclonal antibody.