Rabbit EAF2 Antibody

SKU: orb1526506

Description

EAF2 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended. orb1526506 has not performed satisfactorily when used for WB of EAF2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of EAF2.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 210 and C-terminus
• Target: EAF2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BM040; ELL-associated factor 2; testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; U19

Detection of human EAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EAF2 antibody orb1526506 used for IP at 3 µg/mg lysate.

Detection of human EAF2 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-EAF2 (Cat. No. orb1526506) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

EAF2

UniProt

Q96CJ1

RefSeq (Protein):NP_060926.2