Rabbit anti-CD48 Recombinant Monoclonal Antibody

• Catalog Number: orb1784565
• Category: Antibodies
• Description: Rabbit anti-CD48 Recombinant Monoclonal Antibody
• Target: CD48
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Whole IgG
• Concentration: 250 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Immunogen: Residues 23-216 (ECD)
• UniProt ID: P18181
• Tested applications: ICC, IHC, WB
• Dilution range: WB: 1:1000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR275L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BLAST-1; HM48-1; SLAMF2; SLAM family member 2; sig
• Note: For research use only

Detection of mouse CD48 by western blot. Samples: Whole cell lysate (25 µg) from 70Z/3, WEHI-231, CTLL-2, A20, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD48 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD48 by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.