Rabbit CD48 Recombinant Monoclonal Antibody

SKU: orb1784565

Description

Rabbit CD48 Recombinant Monoclonal Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : WB , ApplicationDilutionRange : 1:1000
• Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR275L
• Immunogen: Residues 23-216 (ECD)
• Target: CD48
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 250 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AI449234;AW610730;BCM1;Bcm-1;BCM1 surface antigen;BLAST;BLAST1;BLAST-1;CD antigen CD48;CD48 antigen;HM48-1;MEM-102;MRC OX-45 surface antigen;Sgp-60;signaling lymphocytic activation molecule 2;SLAM family member 2;SLAMF2

Detection of mouse CD48 by western blot. Samples: Whole cell lysate (25 µg) from 70Z/3, WEHI-231, CTLL-2, A20, and EL4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD48 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD48 by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-CD48 recombinant monoclonal antibody (orb1784565). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

CD48

UniProt

P18181

RefSeq (Protein):NP_031675.1