Rabbit CD47 Recombinant Monoclonal Antibody

SKU: orb1519668

Description

CD47 Antibody

Research Area

Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR131H
• Immunogen: Residues 19-142 (ECD)
• Target: CD47
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

antigen identified by monoclonal antibody 1D8; antigenic surface determinant protein OA3; CD antigen CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 glycoprotein; IAP; integrin associated protein; Integrin-associated protein; integrin-associated signal transducer; leukocyte surface antigen CD47; MER6; OA3; Protein MER6; Rh-related antigen

Detection of human CD47 by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD47 recombinant monoclonal antibody (orb1519668) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Quick Database Links

Gene Symbol

CD47

UniProt

Q08722

RefSeq (Protein):NP_942088.1