Rabbit anti-CD44 Recombinant Monoclonal Antibody

SKU: orb1519905

Description

CD44 Antibody

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR038F
• Immunogen: Between 692 and C-terminus
• Target: CD44

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 50 µg/ml
• Disclaimer: For research use only

Alternative Names

MDU2; hyaluronate receptor; IN; Indian blood group antigen; LHR; MC56; MDU3; MIC4; Pgp1; HUTCH-I; PGP-I; phagocytic glycoprotein 1; Phagocytic glycoprotein I; PGP-1; chondroitin sulfate proteoglycan 8; homing function and Indian blood group system; soluble CD44; CD antigen CD44; CD44 antigen; cell surface glycoprotein CD44; CSPG8; ECMR-III; epican; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCELL; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; Hermes antigen; CDW44

Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, 786-O, HEK293T, A-549, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of mouse CD44 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD44 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of mouse CD44 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human CD44 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used for IP at 20 µl/mg lysate.

Detection of human CD44 in FFPE 786-O cells by ICC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Quick Database Links

Gene Symbol

CD44

UniProt

P16070

RefSeq (Protein):NP_000601.3