Rabbit anti-CD4 Recombinant Monoclonal Antibody

• Catalog Number: orb1519966
• Category: Antibodies
• Description: CD4 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BL-155-1C11
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 26-397 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 6 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: CD4
• UniProt ID: P01730
• NCBI: NP_000607.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: CD4mut; T-cell surface antigen T4/Leu-3; T-cell su
• Background: CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human CD4 by western blot. Samples: Whole cell lysate (50 µg) from U-937, HEK293T, MOLT-4, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody [BL-155-1C11] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody

Detection of human CD4 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG

Detection of human CD4 (shaded) in THP-1 cells by flow cytometry. Antibody: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG