Rabbit BRD4 Recombinant Monoclonal Antibody

SKU: orb1519996

Description

BRD4 Antibody

Images & Validation

• Tested Applications: ChIP, FC, ICC, IHC, IP, WB
• Dilution Range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ICC-IF - 1:25 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BL-149-2H5
• Immunogen: Between 1312 and C-terminus
• Target: BRD4
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1

Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] (orb1519996) used for IP at 20 µl/mg lysate.

Quick Database Links

Gene Symbol

BRD4

UniProt

O60885

RefSeq (Protein):NP_490597.1