Rabbit A-RAF Antibody

SKU: orb1525454

Description

A-RAF Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 150 and 200
• Target: A-RAF
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; ARAF1; Oncogene ARAF1; PKS2; Proto-oncogene A-Raf; proto-oncogene A-Raf-1; proto-oncogene Pks; RAFA1; Ras-binding protein DA-Raf; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein

Detection of human A-RAF by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-A-RAF antibody orb1525454 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. A-RAF was also immunoprecipitated by rabbit anti-A-RAF antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Quick Database Links

Gene Symbol

A-RAF

UniProt

P10398

RefSeq (Protein):NP_001645.1