qPCR MultiplexMaster

SKU: orb2646475

Description

qPCR MultiplexMaster is designed for quantitative real-time analysis of DNA samples using Dual Labeled Fluorescent Probes, e.g. TaqMan, Molecular Beacons or FRET probes. The master mix is specially optimized for setting-up multiplex assays with ≥4 target sequences in a single tube.\nThe system overcomes multiplex limitations of conventional qPCR probe mixes combining an above-average robustness for a multitude of known PCR inhibitors with an excellent sensitivity for amplification of lowest template amounts. \nThe 2x concentrated master mix contains all reagents required for qPCR (except template and primer/probe sets) including a highly processive antibody-inhibited hot-start polymerase and ultra-pure dNTPs.\nThe mixe provides an extremely stringent automatic hot-start allowing reaction set-up and allows temporary storage at room temperature prior to PCR. \nThe reaction chemistry of the mix is optimized for block-based PCR instruments. The mix can also be used with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.

Key Properties

• Concentration: 2x conc.

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles. Storage at 4 °C for up to 1 week
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Master mix for multiplex real-time PCR, 2 x conc. master mix