PMS2 Antibody

SKU: orb344424

Description

PMS2 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-PMS2 antibody has been tested for use in ELISA, western blotting, and SDS-Page. This product is suitable for immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1204
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.
• Purity: This is an protein A purified antibody from ascites fluid directed against PMS2-134 and reacts with full length version of PMS2 in human and hamster tissues. The epitope was putatively mapped to amino acids 58-81 of human PMS2. BLAST analysis indicates that this sequence is 100% identical for human, mouse, rat and chimpanzee. No specific information is available for reactivity with PMS2 protein from other sources.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-PMS2 antibody, DNA mismatch repair gene homologue antibody, H_DJ0042M02.9 antibody, HNPCC4 antibody, PMS 2 antibody, PMS1 protein homolog 2 antibody, PMS2 postmeiotic segregation increased 2 antibody, PMS2CL antibody, PMSL2 antibody

Relevant protein domains for human PMS2.

Western blot analysis is shown using Biorbyt's Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5, 10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by washing. A 1:20000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results.

Quick Database Links

UniProt

P54278

RefSeq:P54278.2