Pig Immunoglobulin M (IgM) ELISA Kit

SKU: orb781450

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin M(IgM).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IgM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Porcine
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 µg/mL
• Sensitivity: 14.3 ug/mL
• Concentration: 2000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IGHM ELISA Kit, Immunoglobulin Heavy Constant Mu ELISA Kit, Ig mu chain C region ELISA Kit

References

Can Ning 1, Wenguang Xiao 1, Zengenni Liang 2, You Wu 1, Hui Fan 1, Siqi Wang 1, Xiangyi Kong 1, Yongkang Wang 1, Aoao Wu 1, Yuanyuan Li 1, Zhihang Yuan 1, Jing Wu 3, Chenglin Yang Melatonin alleviates T-2 toxin-induced oxidative damage, inflammatory response, and apoptosis in piglet spleen and thymus Int Immunopharmacol ., (2024)

PubMed: 38354511

Applications

ELISA

Reactivity

Porcine