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Phospho-V-Myb+C-Myb (Ser11) Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb1499349
| Catalog Number | orb1499349 |
|---|---|
| Category | Antibodies |
| Description | Phospho-V-Myb+C-Myb (Ser11) Recombinant Rabbit Monoclonal Antibody |
| Target | MYB |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthesised phosphopeptide derived from human MYB around the phosphorylation site of Ser11 |
| UniProt ID | P10242 |
| MW | 84 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:100-500, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:50-200, IF=1:50-200 |
| Antibody Type | Recombinant Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Avian myeloblastosis viral(v-myb) oncogene homolog Read more... |
| Note | For research use only |
ICC staining of P-V-Myb+C-Myb (S11) in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499349, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-P-V-Myb+C-Myb (S11) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1499349, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
