Phospho-SMAD3(S213) Antibody

SKU: orb1931224

Description

Phospho-SMAD3(S213) Antibody

Research Area

Cancer Biology, Infectious Disease & Virology, Metabolism Research, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: IHC-P - 1:100-500, WB - 1:1000
• Reactivity: Human
• Predicted Reactivity: Gallus, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 48081 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MADH3

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-Phospho-SMAD3-S213 Pab is used in Western blot to detect Phospho-SMAD3-S213 in Ramos tissue lysate

All lanes: Anti-SMAD3 (S213) Antibody at 1:1000 dilution. Lane 1: HACAT whole cell lysate. Lane 2: HT-1080 whole cell lysate. Lane 3: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48, 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P84022