Phospho-PI 3 Kinase p110 delta (Tyr524) Rabbit Polyclonal Antibody

SKU: orb6720

Description

Phospho-PI 3 Kinase p110 delta (Tyr524) Rabbit Polyclonal Antibody

Research Area

Lipid Signaling, Signal Transduction, TLR Signaling Pathway

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PIK3CD around the phosphorylation site of Tyr524 EL(p-Y)EH
• Target: PIK3CD
• Molecular Weight: 115 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PI 3 Kinase p110 delta(phospho Tyr524); PIK3CD(phospho Y524); PI3 Kinase p110 delta; p110D; p110delta; Phosphatidylinositol 3 kinase catalytic delta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform; Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform; phosphoinositide 3 kinase B; phosphoinositide 3 kinase C; phosphoinositide 3 kinase catalytic delta polypeptide; PI3 kinase p110 subunit delta; PI3K; PIK3CD; PK3CD; PtdIns 3 kinase p110; PI 3 Kinase p110 delta.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-phospho-PI 3 Kinase p110 delta (Tyr524) antibody (orb6720), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Rat Thymus Lysates, Lane 2: Human HT-1080 cell Lysates, Primary: Anti-phospho-PI 3 Kinase p110 delta (Tyr524) (orb6720) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115kDa, Observed band size: 115 kDa.

Tissue/Cell: mouse spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-PI 3 Kinase p110 delta (Tyr524) Polyclonal Antibody, Unconjugated (orb6720) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PIK3CD

UniProt

O00329