Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2561184
• Category: Antibodies
• Description: Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 9G6
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PAK1 around the phosphorylation site of S144
• Antibody Type: Recombinant Antibody
• Dilution range: WB=1:500-1:5000, IHC-P=1:100-1:200, IHC-F=1:100-1:200, ICC/IF=1:50-1:200, IF=1:100-1:200, IP=1:20-1:50, Flow-Cyt=1:20-1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: PAK1
• UniProt ID: Q13153
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% sodium azide and 50% glycerol.
• Alternative names: ADRB2; alpha-PAK; CDC42/RAC effector kinase PAK-A;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa Cell lysate treated with lambda phosphatase. Using Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) (orb2561184) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.