Phospho-P53(S9) Antibody

SKU: orb1931241

Description

Phospho-P53(S9) Antibody

Research Area

Cancer Biology, Cell Biology, Infectious Disease & Virology, Microbiology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: DOT, IF, WB
• Dilution Range: DB - 1:500, WB - 1:1000, IF - 1:10-50
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Dot blot analysis of anti-Phospho-P53-S9 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.

Confocal immunofluorescent analysis of Phospho-P53-S9 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of Phospho-P53-S9 in HL60 (left) and Ramos (right) cell line lysate.

Quick Database Links

UniProt

P04637