Phospho-p107 (Thr369) Rabbit Polyclonal Antibody

SKU: orb100309

Description

Phospho-p107 (Thr369) Rabbit Polyclonal Antibody

Research Area

Cancer Research, Nuclear Signaling Pathway, RB Family, Tumor Suppressors

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human RBL1 around the phosphorylation site of Thr369 PS(p-T)PL
• Target: RBL1
• Molecular Weight: 121 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p107(phospho T369); p-p107(phospho T369); RBL1(phospho T369); 107 kDa retinoblastoma-associated protein; Cellular protein 107; Cellular protein p107; CP107; MGC40006; p107; PRB1; RBL1; RBL1_HUMAN; Retinoblastoma like protein 1; retinoblastoma-like 1(p107); Retinoblastoma-like protein 1.

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-phospho-p107 (Thr369) antibody (orb100309), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-APC, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with phospho-p107 (Thr369) Polyclonal Antibody, Unconjugated (orb100309) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Lane 5: Human 293T cell lysates, Lane 6: Human U-2 OS cell lysates, Primary: Anti-phospho-p107 (Thr369) (orb100309) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 121 kDa, Observed band size: 130 kDa.

Quick Database Links

Gene Symbol

RBL1

UniProt

P28749