Phospho-Jak1 (Tyr1034 + Tyr1035) Rabbit Polyclonal Antibody

• Catalog Number: orb6249
• Category: Antibodies
• Description: Phospho-Jak1 (Tyr1034 + Tyr1035) Rabbit Polyclonal Antibody
• Target: JAK1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035 KE(p-Y)(p-Y)TV
• UniProt ID: P23458
• RRID: AB_10941544
• MW: 133 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg /Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: JAK1(phospho Y1034+Y1035); p-JAK1(phospho Y1034+Y1
• Research Area: Cell Biology, JAK/ STAT Signaling Pathway, Kinases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-Phospho-Jak1 antibody (orb6249), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Publition Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes, IF: 2.5.

Sample: A549 (Human) cell Lysate at 40 ug, Primary: Anti-p-Jak1 (Tyr1034 + Tyr1035) (orb6249) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 133kD, Observed band size: 133kD.