Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559184
• Category: Antibodies
• Description: Phospho-IRF3 (Ser386) Recombinant Rabbit Monoclonal Antibody
• Target: IRF3
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human IRF3 around the phosphorylation site of Ser386 S(p-S)LE
• UniProt ID: Q14653
• MW: 47 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-1000, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 28C3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRF3(S386); IRF3(phospho S386); IRF3(phospho Ser38
• Research Area: Immunology, SARS /COVID-19, TLR Signaling Pathway
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-IRF3 (S386)) monoclonal Antibody, Unconjugated (orb559184) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.