Phospho-HS1 (Tyr397) (F12) rabbit mAb FITC Conjugate Antibody

SKU: orb1946042

Description

HS1 is expressed in lymphoid and hematopoietic cells, and is heavily post-translationally modified. HS1 deficient mouse models have demonstrated the protein's role in receptor-mediated apoptosis and proliferation. HS1 is phosphorylated at Tyr378 and Tyr397 by the kinase Syk, providing a high-affinity binding site for SH2 domains from the Src family. Following this interaction, HS1 is then phosphorylated at Tyr222 by c-Fgr, Lyn, and Fyn kinases. HS1 plays an important role in T cell signaling, where HS1 phosphorylation recruits and activates Vav1 at the immune synapse. As a homolog of the actin binding protein cortactin, HS1 has been shown to mediate neutrophil chemotaxis through phosphorylation of tyrosines 222, 378, and 397.

Images & Validation

• Tested Applications: FC
• Dilution Range: For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: HS1Y397-F12
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1
• Purification: Protein A+G
• Conjugation: FITC

Storage & Handling

• Storage: 2-8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS, 0.09% NaN3, 0.2% rAlbumin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1

Flow cytometric analysis of Ramos cells untreated and unstained as negative control (blue) or untreated (red) or treated with pervanadate (green) and stained using Phospho-HS1 (Tyr397) FITC conjugated antibody HS1Y397-F12. Cat. #orb1946042.

Quick Database Links

UniProt

P14317