Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb783424
• Category: Antibodies
• Description: Phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Recombinant Rabbit Monoclonal Antibody
• Target: GSK3B
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GSK-3 Beta around the phosphorylation site of Tyr216
• UniProt ID: P49841
• MW: 47/51 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 5G8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: phospho-GSK3(alpha+beta)(Y216+Y279); GSK3B(phospho
• Research Area: Cancer Research, Cardiovascular Research, Cell Bio
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Phospho-GSK3 (alpha+beta) (Y216+Y279)) monoclonal Antibody, Unconjugated (orb783424) 1:50, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-GSK3 Alpha + Beta (Tyr279+Tyr216)) Monoclonal Antibody, Unconjugated (orb783424) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human 293T cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human MCF-7 cell lysates, Primary: Anti-phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) (orb783424) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47/51 kDa, Observed band size: 47, 51 kDa.