Phospho-ERK1 + 2 (Thr183/Tyr185) Rabbit Polyclonal Antibody

• Catalog Number: orb10606
• Category: Antibodies
• Description: Phospho-ERK1 + 2 (Thr183/Tyr185) Rabbit Polyclonal Antibody
• Target: MAPK3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185 FL(p-T)E(p-Y)V
• RRID: AB_10746449
• MW: 42/44 kDa
• Tested applications: ICC
• Dilution range: ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERK1+ERK2(phospho Thr183/Tyr185); phospho-ERK1/MAP
• Research Area: Cell Biology, Cytoplasmic, MAPK/ERK Signalling Pat
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhao, Haiyan et al. Inhibition of endocan attenuates monocrotaline-induced connective tissue disease related pulmonary arterial hypertension Int Immunopharmacol, 42, 115-121 (2017)

PubMed: 27912147

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) polyclonal Antibody, Unconjugated (orb10606) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-ERK1 + 2 (Thr183/Tyr185)) Polyclonal Antibody, Unconjugated (orb10606) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.