Phospho-CDK9 (Thr186) Rabbit Polyclonal Antibody

• Catalog Number: orb5836
• Category: Antibodies
• Description: Phospho-CDK9 (Thr186) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human CDK9 around the phosphorylation site of Thr186 RY(p-T)NR
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: CDK9
• UniProt ID: P50750
• RRID: AB_10939592
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CDK9_HUMAN; Cyclin-dependent kinase 9; EC:2.7.11.2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Brain (Mouse) Lysate at 40 ug, Primary: Anti-p-CDK9 (Thr186) (orb5836) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Sample: Lane 1: Human 293T cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human HepG2 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human A431 cell lysates, Primary: Anti-Phospho-CDK9 (Thr186) (orb5836) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kDa, Observed band size: 42, 55 kDa.

Tissue/Cell: human laryngeal tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Phospho-CDK9 (Thr186) Polyclonal Antibody, Unconjugated (orb5836) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.