Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody

• Catalog Number: orb156346
• Category: Antibodies
• Description: Phospho-c-Fos (Ser362) Rabbit Polyclonal Antibody
• Target: FOS
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human c-Fos around the phosphorylation site of Ser362 KG(p-S)SS
• UniProt ID: P01100
• MW: 41 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: c-Fos(phospho S362); c-Fos(phospho-Ser362); c-Fos(
• Research Area: Cancer Research, DNA and RNA Research, Neuroscienc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin embedded, and decalcified in EDTA mouse osteosarcoma labeled with Anti-Phospho-c-Fos (Ser362) Polyclonal Antibody, Unconjugated (orb156346) at 1:100 followed by conjugation to the secondary antibody and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-c-Fos (Ser362)) Polyclonal Antibody, Unconjugated (orb156346) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Cerebrum (Rat) Lysate at 40 ug, A431 (Human) Cell Lysate at 30 ug, Adrenal glands (Mouse) Lysate at 40 ug, Adrenal glands (Rat) Lysate at 40 ug, Uterus (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62/46 kD, Observed band size: 46 kD.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-c-Fos (Ser362) (orb156346) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kD, Observed band size: 41 kD.