Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559194
• Category: Antibodies
• Description: Phospho-AMPK alpha 1 (Ser496) Recombinant Rabbit Monoclonal Antibody
• Target: PRKAA1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse AMPK alpha 1 around the phosphorylation site of Ser496 SG(p-S)VS
• UniProt ID: Q5EG47
• MW: 64 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 11G1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: p-AMPK alpha 1(S496); phospho-AMPK alpha-1(S496);
• Research Area: Alzheimer's disease, Cancer Research, Cell Biology
• Note: For research use only

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Huvec (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AMPK alpha 1 (Ser496) (orb559194) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kD, Observed band size: 60 kD.