PGP9.5 / UchL1 Antibody

SKU: orb639550

Description

This mAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rUBCE-L1
• Immunogen: Recombinant human protein was used as the immunogen for the recombinant UchL1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human cerebellum with recombinant UchL1 antibody (clone rUBCE-L1). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of permeabilized human T98G cells with recombinant UchL1 antibody cocktail (green, clone rUBCE-L1) and Nucspot (red).

Western blot testing of human brain lysate with recombinant UchL1 antibody (clone rUBCE-L1). Predicted molecular weight ~25 kDa.

Flow cytometry testing of permeabilized human T98G cells with recombinant UchL1 antibody (clone rUBCE-L1); Red=isotype control, Blue= recombinant PGP9.5 antibody.

Quick Database Links

UniProt

P09936