Peroxiredoxin 1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb544576
• Category: Antibodies
• Description: Peroxiredoxin 1 Recombinant Rabbit Monoclonal Antibody
• Target: PRDX1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Peroxiredoxin 1 protein, full length
• UniProt ID: Q06830
• MW: 22 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Antibody Type: Primary Antibody
• Clone Number: 7A1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; P
• Research Area: Inflammation, Inflammatory Mediators, Oxidative St
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Peroxiredoxin 1) Polyclonal Antibody, Unconjugated (orb544576) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, U2OS (Human) Cell Lysate at 30 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Peroxiredoxin 1 (orb544576) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 22 kD.