PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody

SKU: orb751161

Description

Mouse monoclonal antibody against CD274 conjugated to Biotin

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: Flow Cytometry (1-2ug/million cells); ,Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human, Mouse
• Application Notes:
  Positive Control: Jurkat, RAW, HEK293 or HepG2 cells. Human lung SqCC, cervical or breast carcinoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PDL1/2746
• Immunogen: Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
• Target: CD274
• Source/Expression System: Bioreactor
• Molecular Weight: 37-50kDa
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-B7 homolog 1 antibody, anti-B7-H1 antibody, anti-CD274 antibody, anti-PD-L1 antibody, anti-PDCD1 ligand 1 antibody, anti-PDCD1L1 antibody, anti-PDCD1LG1 antibody, anti-Programmed cell death 1 ligand 1 antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-mouse IgG-CF488 (green). Nuclei are stained with RedDot (red).

Flow Cytometric Analysis of human Jurkat cells using PE-conjugated PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (blue); isotype control (red).

Formalin-fixed, paraffin-embedded human basal cell carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human breast carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human cervical carcinoma stained with PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746). HIER: Tris/EDTA, pH9.0, 45min. 2°: HRP-polymer, 30min. DAB, 5min.

Immunofluorescent staining of Jurkat cells. PD-L1-Monospecific Mouse Monoclonal Antibody (PDL1/2746) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746) (unconjugated). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified PD-L1 Mouse Monoclonal Antibody (PDL1/2746). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

CD274

UniProt

Q9NZQ7

NCBI Gene ID

29126