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Description
Research Area
Images & Validation
−| Tested Applications | FC, IHC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; IHC: 1/100 - 1/500; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rabbit, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7H4F8 |
| Immunogen | Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli. |
| Target | Disease |
| Molecular Weight | 28.7kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot analysis of A431 (Lane 1), HEK293 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Raji (Lane 5), MOLT4 (Lane 6), COS7 (Lane 7), MCF-7 (Lane 8) cell lysate using PCNA antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of PCNA antibody

Flow cytometric analysis of MOLT4 cells lysates using PCNA antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using PCNA antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded colon cancer tissues using PCNA antibody

Western blot analysis of human PCNA using PCNA antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using PCNA antibody
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PCNA (orb197875)
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